Simultaneous differentiation and typing of Entamoeba histolytica and Entamoeba dispar.

تمایز انتاموبا هیستولیتیکا و انتاموبا دیسپار با یک واکنش زنجیره‌ای پلی‌مراز

Abstract: Background and Aim: In most part of the world detection of cysts and trophozoites of Entamoeba is based on morphological structure of this species in stool sample by microscopy. However, microscopic examination is unable to distinguish between similar morphological protozoa such as Entamoeba histolytica and Entamoeba dispar. A simple and cost-effective method is needed in medical lab...

PRELIMINARY COMPARATIVE STUDY OF ENTAMOEBA HISTOLYTICA AND ENTAMOEBA DISPAR BY PCR TECHNIQUE IN IRAN

It has been suggested that two distinct species exist within what was originally known as Entamoeba histolytica and E. dispar. These are Entamoeba dispar for the nonpathogenic and E. histolytica for the pathogenic fonn. Differentiation of these two organisms is of great clinical importance since they are morphologically indistinguishable and both fonns can infect the human intestinal cavity...

Importance of differentiation of Entamoeba histolytica from entamoeba dispar.

روش های های رایج و نوین تشخیص آزمایشگاهی بیماری آمیبیاز

The genus Entamoeba contains many species, six of which (Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, Entamoeba polecki, Entamoeba coli, and Entamoeba hartmanni) reside in the human intestinal lumen. Entamoeba histolytica is the causative agent of amebiasis in humans with a worldwide distribution. Correct detection of E.histolytica is frequently a major concern in diagnostic ...

Differentiation of Entamoeba histolytica and Entamoeba dispar by PCR: a preliminary study in Izmir, Turkey.

The causative agent of amoebiasis is currently attributed to two distinct species (E. histolytica and E. dispar). The aim of this study was to differentiate these species by PCR in stool samples. Isolated genomic DNA was amplified by PCR and band products of 101 bp (E. dispar) were obtained. All seven stool samples were found to be E. dispar, not E. histolytica. Our results demonstrated the sig...